Advance your epigenetics research with NGS methods.

In a nutshell
- Learn how bisulfite sequencing works
- Understand how bisulfite-treated reads are mapped to a reference genome
- Perform basic analyses (call methylated regions, perform basic downstream analyses)
- Use shell scripting to create reusable data pipelines
- Visualize results (ready-to-publish)

The purpose of this workshop is to get a deeper understanding of the use of bisulfite-treated DNA in order to analyze the epigenetic layer of DNA methylation. Advantages and disadvantages of the so-called 'bisulfite sequencing' and its implications on data analyses will be covered. The participants will be trained to understand bisulfite-treated NGS data, to detect potential problems/errors and finally to implement their own pipelines. After this course they will be able to analyze DNA methylation and create ready-to-publish graphics.

By the end of this workshop the participants will:
- be familiar with the sequencing method of Illumina
- understand how bisulfite sequencing works
- be aware of the mapping problem of bisulfite-treated data
- understand how bisulfite-treated reads are mapped to a reference genome
- be familiar with common data formats and standards
- know relevant tools for data processing
- automate tasks with shell scripting to create reusable data pipelines
- perform basic analyses (call methylated regions, perform basic downstream analyses)
- plot and visualize results (ready-to-publish)
- be able to reuse all analyses