This practical aims to familiarize you with the Galaxy user interface & execute a complete analysis of RNA-seq data.

It will teach you how to perform basic tasks such as importing data, running tools, working with histories, creating workflows, and sharing your work. In this tutorial we will introduce you to bare basics of Galaxy:

Getting data from external databases such as UCSC
Performing simple data manipulation
Understanding Galaxy’s History system
Creating and running a workflow

The RNA-seq data analysis part comprises:

Filtering and quality control of reads
Mapping of reads to reference genomes
Examples of downstream data-analysis possibilities (variant calling, differential expression, etc.) 

This workshop is organised by ELIXIR Belgium.