using next generation DNA sequencing. The principal advantage of RAD-seq is the ability to simultaneously examine tens of thousands of genetic loci with vastly reduced sequencing costs versus whole genome approaches. RAD-seq can be used on crosses of any design as well as wild populations and organisms with and without a reference genome. Applications include SNP discovery, linkage mapping and QTL mapping, but also more complex analyses such as population genomic and phylogeographic studies. The aim of this workshop is to familiarise researchers with RAD-seq data and to initiate them in the analysis by providing lectures and practicals on analysis methodologies. In the practicals Illumina-generated sequencing data and various widely used software programs (e.g. FastQC, BWA, samtools, IGV, Stacks) will be used. In the last section of this workshop we will focus on linkage analysis and population genomics.