The training will start with an introduction on the available recombinant protein expression systems, with their advantages and limitations, from which to choose for your favorite protein. Also, high-throughput protein expression, expression in plant cells, antibody and nanobody expression and a difficult-to-express protein case will be discussed. A next session will focus on strategy design to optimize protein purification and how to address quality assurance of the purified proteins. For clinical use of a protein-based biological stability and production yields are critical issues that come on top of the desired therapeutic efficacy. Why and how to deal with these critical aspects will be addressed as well during the training. Furthermore, also the key steps that are required for optimal protein formulation will be reviewed. We plan to end the day with a keynote talk from a pharma industry speaker illustrating the issues that need to be considered when engineering recombinant proteins for clinical applications.