This course will provide an introduction to next generation sequencing (NGS) platforms, data analysis and tools for data quality control, read alignment (mapping), differential expression, de novo and referenced based transcriptome assembly, annotation and gene discovery of RNA sequencing (RNA-Seq) data. The course will be delivered using a mixture of lectures and computer based hands on practical sessions using real data.
This course is aimed at wet-lab biologists who are involved in research projects that will require the handling and analysis of NGS data.

A significant proportion of the course will be computer-based using command line tools in the Unix environment, therefore, in order to gain maximum benefit from the course all attendees will be required to have basic Unix skills. This means applicants must at a minimum be able to move easily around the file system and make directories (e.g. pwd, cd, ls, mkdir), manage files (e.g. cp, mv, ln, rm, less, head, tail, wc) and search with grep.