Date: 27 - 29 September 2017

Timezone: Amsterdam

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Next-generation sequencing (NGS) of RNA libraries (RNA-Seq) has become increasingly common and it largely replaced microarray technology for gene expression profiling. The aim of this course is to get a deeper understanding of RNA-Seq experiments, providing a theoretical introduction to the data processing steps, together with practical sessions illustrating the use of the most popular data analysis tools. The classroom size is limited to 25 participants to optimize the learning and the interaction with the instructors. Starting from the raw sequenced data coming from different phenotypical samples (e.g disease vs healthy control samples), genes which are differentially expressed between the two conditions are determine. Some strategies are illustrated for detecting alternative splicing products, predicting novel isoforms and gene fusion events. Some methods for downstream analysis are described to give insight into how biological knowledge can be generated from RNA-Seq experiments. A lecture on single-cell RNA-Seq will provide an idea of how transcriptome data from individual cells is now emerging as a powerful tool, allowing the study of cell-to-cell gene expression heterogeneity in the same tissue.

Contact: elixir.ita.training@gmail.com, amarabotti@unisa.it

Keywords: NGS, RNA-Seq, Bioinformatics, Data analysis, High Throughput Sequencing Analysis, Gene Expression

Venue: Campus di Fisciano, Università degli Studi di Salerno, Via Giovanni Paolo II, n. 132

City: Fisciano

Region: Provincia di Salerno

Country: Italy

Postcode: 84084

Organizer: Anna Marabotti (University of Salerno and ELIXIR-IIB Training Team, Italy), Roberto Tagliaferri (University of Salerno, Italy), Alessandro Weisz (University of Salerno, Italy), Loredana Le Pera (ELIXIR-IIB Training Team and IIT, Italy), Allegra Via (ELIXIR-IIB Training Coordinator, CNR-IBPM, Italy)

Host institutions: Campus di Fisciano, University of Salerno, Italy

Eligibility:

  • First come first served
  • Registration of interest

Target audience: PhD students and young researchers in the life science and computational biology field who are planning to use RNA-seq data and are looking for the best practices to analyze these types of data

Capacity: 25

Event types:

  • Workshops and courses

Sponsors: ELIXIR Italy

Scientific topics: Gene expression, RNA-Seq, Bioinformatics, Sequencing, RNA splicing, Transcriptomics


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