Single cell RNA sequencing (scRNA-seq) offers great potential, and new experimental technologies and data analysis methods have been developed at rapid pace. However, these fast developments put high pressure on life scientists to keep up with the challenging demands of data analysis, and the need for training is enormous.This GOBLET-ELIXIR workshop brought together scRNA-seq trainers to share experiences, challenges and best practices. The presentation videos are available in YouTube.Welcome, Eija Korpelainen, CSCThe Swedish efforts in teaching single cell bioinformatics, Paulo Czarnewski (ELIXIR-SE, NBIS, Sweden)Flipped classroom approach and Chipster in scRNA-seq training, Eija Korpelainen (ELIXIR-FI, CSC, Finland)eLearning in scRNA-seq training, Maria Lehtivaara (ELIXIR-FI, CSC, Finland)Galaxy and scRNA-Seq training, Pavankumar Videm (ELIXIR-DE, Galaxy, Germany)scRNAseq hands on (Italy, EMBL, Singapore), Raffaele Calogero (ELIXIR-IT, University of Torino, Italy)Single-cell RNA seq training in the Netherlands, Ahmed Mahfouz (ELIXIR-NL, LUMC, Netherlands)Organizing advanced single-cell courses, Charlotte Soneson (ELIXIR-CH, SIB, Switzerland)Single-cell RNA-seq training at Cambridge, Hugo Tavares (ELIXIR-UK, University of Cambridge, UK)