This course was designed to provide basic knowledge on the use of the biological model zebrafish with main emphasis on its use in the context of the study of human diseases and biomedical sciences. Introduction to genetic manipulation and production of transgenic lines and "knock-out". It is intended to transfer skills for manipulation and use of the zebrafish in genetic manipulation techniques, applications of transgenics and mutants for study of phenotypes of interest, microinjection and studies of tissue regeneration.