Many bioinformatics tools have been developed over the last decennium, most of which were meant to be used in the lab of the life scientist. Unfortunately, only a few made it, because the tools are often too non-intuitive and too much a black box to the life scientist. Therefore Hanka Venselaar developed HOPE to at least make the explanation of the phenotypic effect of mutations in (human) proteins intuitive for the non-bioinformatics expert. We developed this course to explain the basics of this program, and the underlying theory.