RNA-Seq Analysis
This course builds on the core skills introduced in the Introduction to R, Introduction to Unix and Introduction to SeqMonk courses to provide a more in depth look at the analysis of RNA-Seq data. The course starts with a comprehensive lecture covering the theory of RNA-Seq data generation and analysis and is then followed by a long hands-on practical session which runs though the entire RNA-Seq analysis pipeline from raw fastq files to a list of differentially expressed candidate genes.
Course Content
- The theory of RNA-Seq analysis
- Raw data QC
- Mapping RNA-Seq data with tophat
- Viewing RNA-Seq data with SeqMonk
- Differential expression analysis with DESeq
- Reviewing and visualising differential expression hits
Course Material
Scientific topics: RNA-Seq
Activity log
