This Spring School will focus on theoretical and practical aspects of lipidomics, using state-of-the-art approaches. There will be a major focus on the underlying biology and biochemistry of lipids, including their roles in health and disease. There will be 20+ sessions over 5 days, topics include neutral lipids, fatty acids, eicosanoids, sphingolipids, sterols and bile acids. An introduction to MS-based lipidomics will cover all aspects from sample preparation, storage, chromatography, instrumentation, and identification using databases including LIPID MAPS. There will be a dedicated session on novel technologies, such as iKnife, REIMS, imaging, and new standards for quantitation. This will be alongside practical tutorials on software and informatics tools for lipidomics. An introduction into data integration using biostatistics and systems biology, to include lipid