Single cell analysis
Gone is the pre-annotated, high quality tutorial data - now you have real, messy data to deal with. You have decisions to make and parameters to decide. This learning pathway challenges you to replicate a published analysis as if this were your own dataset. You will be introduced to a few more tools available for scRNA-seq in Galaxy. Finally, if our tool offerings are not enough for you, you will be directed towards how to use coding notebooks within Galaxy, setting you up to analyse scRNA-seq in R or python notebooks.
The data is messy. The decisions are tough. The interpretation is meaningful. Come here to advance your single cell skills! Note that you get two options for inferring trajectories.
For support throughout these tutorials, join our Galaxy single cell chat group on Matrix to ask questions!
New to Galaxy and/or the field of scRNA-seq? Follow this learning path to get familiar with the basics!
Keywords: test, GTN
Contributors: Munazah Andrabi
Status: Under development
Target audience: Undergraduate students
Learning objectives:
- Generate a cellxgene matrix for droplet-based single cell sequencing data
- Interpret quality control (QC) plots to make informed decisions on cell thresholds
- Find relevant information in GTF files for the particulars of their study, and include this in data matrix metadata
Module 1: Case study
• beginner 3 materials-
e-learning
Generating a single cell matrix using Alevin
• beginner10x MIGHTS Single Cell paper-replication -
e-learning
Combining single cell datasets after pre-processing
• beginner10x MIGHTS Single Cell paper-replication -
e-learning
Filter, plot and explore single-cell RNA-seq data with Scanpy
• beginner10x MIGHTS Single Cell paper-replication
Module 2: Inferring trajectories
•• intermediate 2 materialsModule 3: Moving into coding environments
••• advanced 3 materials-
e-learning
JupyterLab in Galaxy
• beginnerUsing Galaxy and Managing your Data interactive-tools -
e-learning
Generating a single cell matrix using Alevin and combining datasets (bash + R)
• beginner10x MIGHTS Single Cell jupyter-notebook paper-replication -
e-learning
RStudio in Galaxy
• beginnerUsing Galaxy and Managing your Data interactive-tools
Activity log