slides

An introduction to scRNA-seq data analysis

Abstract

Single-cell RNA-seq

Questions this will address

  • How are samples compared?
  • How are cells captured?
  • How does bulk RNA-seq differ from scRNA-seq?
  • Why is clustering important?

Learning Objectives

  • To understand the pitfalls in scRNA-seq sequencing and amplification, and how they are overcome.
  • Know the types of variation in an analysis and how to control for them.
  • Grasp what dimension reduction is, and how it might be performed.
  • Be familiarised with the main types of clustering techniques and when to use them.

Licence: Creative Commons Attribution 4.0 International

Keywords: Single Cell

Target audience: Students

Resource type: slides

Version: 5

Status: Active

Prerequisites:

Introduction to Galaxy Analyses

Learning objectives:

  • To understand the pitfalls in scRNA-seq sequencing and amplification, and how they are overcome.
  • Know the types of variation in an analysis and how to control for them.
  • Grasp what dimension reduction is, and how it might be performed.
  • Be familiarised with the main types of clustering techniques and when to use them.

Date modified: 2024-08-08

Date published: 2021-01-29

Authors: Mehmet Tekman

External resources:

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