e-learning

Annotating a protein list identified by LC-MS/MS experiments

Abstract

ProteoRE Galaxy instance provides necessary tools to execute a whole annotation pipeline of a protein list identified by LC-MS/MS experiments. This activity introduces these tools and guides you through a simple pipeline using some example datasets based on the study entitled "Proteomic characterization of human exhaled breath condensate".

About This Material

This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.

Questions this will address

  • How to filter out technical contaminants?
  • How to check for tissue-specificity?
  • How to perform enrichment analysis?
  • How to map your protein list to pathways (Reactome)?
  • How to compare your proteome with other studies?

Learning Objectives

  • Execute a complete annotation pipeline of a protein list identified by LC-MS/MS experiments

Licence: Creative Commons Attribution 4.0 International

Keywords: DDA, Proteomics, human

Target audience: Students

Resource type: e-learning

Version: 11

Status: Active

Prerequisites:

Introduction to Galaxy Analyses

Learning objectives:

  • Execute a complete annotation pipeline of a protein list identified by LC-MS/MS experiments

Date modified: 2024-06-14

Date published: 2019-09-12

Authors: David Christiany, Florence Combes, Valentin Loux, Yves Vandenbrouck

Scientific topics: Proteomics


Activity log