DNA sequence data has become an indispensable tool for Molecular Biology & Evolutionary Biology. Study in these fields now require a genome sequence to work from. We call this a 'Reference Sequence.' We need to build a reference for each species. We do this by Genome Assembly. De novo Genome Assembly is the process of reconstructing the original DNA sequence from the fragment reads alone.

Questions of the tutorial:

• I just assembled a genome. How does it compare with already sequenced genomes?
• How do I find rearranged, inserted, or deleted regions?

Objectives of the tutorial:

• Identification of the most closely related genome to my new assembly
• Perform sequence comparison to locate rearrangements
• Identify genes located in deletions