e-learning
Clinical Metaproteomics 2: Discovery
Abstract
This tutorial can be followed with any user-defined database but would work best if the clinical metaproteomics database generation module was used (see Database Generation tutorial). The MetaNovo tool generates a more manageable database that contains identified proteins. The MetaNovo-generated database merged with Human SwissProt (reviewed only) and contaminants (cRAP) databases to generate a compact database (~21.2k protein sequences) that will be used for peptide identification.
About This Material
This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.
Questions this will address
- How to perform database searching?
- How to extract microbial and Human protein and peptide sequences from the results
Learning Objectives
- Perform Database searching using two algorithms
- Extract confident peptides and proteins
- Generate a microbial peptide panel for verification
Licence: Creative Commons Attribution 4.0 International
Keywords: Proteomics, label-TMT11
Target audience: Students
Resource type: e-learning
Version: 4
Status: Active
Prerequisites:
- Introduction to Galaxy Analyses
- Proteomics
Learning objectives:
- Perform Database searching using two algorithms
- Extract confident peptides and proteins
- Generate a microbial peptide panel for verification
Date modified: 2024-12-05
Date published: 2024-02-06
Contributors: Björn Grüning, Bérénice Batut, Helena Rasche, Pratik Jagtap, Saskia Hiltemann, Subina Mehta, Timothy J. Griffin
Scientific topics: Proteomics
Activity log
