e-learning

CUT&RUN data analysis

Abstract

In many organism, transcription factors (TF) play an important tole in the regulation of the gene expression. In human, we have up to 2,800 proteins and more than 1,600 are

About This Material

This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.

Questions this will address

  • Which binding motif has the transcription factor GATA1?
  • What kind of quality control do I have to do?
  • How to analyse and visualise CUT&RUN data?

Learning Objectives

  • Apply appropriate analysis and quality control steps for CUT&RUN
  • Apply an enrichment analysis and a robust peak detection
  • Find protein binding motifs

Licence: Creative Commons Attribution 4.0 International

Keywords: Epigenetics

Target audience: Students

Resource type: e-learning

Version: 2

Status: Draft

Prerequisites:

  • Introduction to Galaxy Analyses
  • Mapping
  • Quality Control

Learning objectives:

  • Apply appropriate analysis and quality control steps for CUT&RUN
  • Apply an enrichment analysis and a robust peak detection
  • Find protein binding motifs

Date modified: 2024-12-11

Date published: 2024-03-06

Authors: Florian Heyl

Contributors: Björn Grüning, Florian Heyl, Helena Rasche, Lucille Delisle, Saskia Hiltemann

Scientific topics: Epigenomics


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