e-learning

DIA Analysis using OpenSwathWorkflow

Abstract

This training covers data independent acquisition (DIA) mass spectrometry (MS) applying spectral libraries for peptide identification and quantification.

About This Material

This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.

Questions this will address

  • How to analyze data independent acquisition (DIA) mass spectrometry (MS) data using OpenSwathWorkflow?
  • How to detect different Spike-in amounts of Ecoli in a HEK-Ecoli Benchmark DIA dataset?

Learning Objectives

  • Analysis of HEK-Ecoli Spike-in DIA data in Galaxy
  • Understanding DIA data principles and characteristics
  • Using OpenSwathworkflow to analyze HEK-Ecoli Spike-in DIA data

Licence: Creative Commons Attribution 4.0 International

Keywords: DIA, Proteomics

Target audience: Students

Resource type: e-learning

Version: 10

Status: Active

Prerequisites:

  • Introduction to Galaxy Analyses
  • Library Generation for DIA Analysis

Learning objectives:

  • Analysis of HEK-Ecoli Spike-in DIA data in Galaxy
  • Understanding DIA data principles and characteristics
  • Using OpenSwathworkflow to analyze HEK-Ecoli Spike-in DIA data

Date modified: 2023-11-09

Date published: 2020-12-02

Authors: Matthias Fahrner, Melanie Föll

Scientific topics: Proteomics


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