e-learning

Formation of the Super-Structures on the Inactive X

Abstract

Within a cell nucleus, the DNA is tightly-packed and the chromatin is spatially distributed with different levels and scales of organizations.

About This Material

This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.

Questions this will address

  • Histone modification?
  • How is a raw set of ChIP-seq data processed and analyzed?
  • Where are the enriched regions for H3K27me3, H3K4me3 and CTCF on the chrX?

Learning Objectives

  • Inspect the read quality
  • Trim low quality bases
  • Map reads on a reference genome
  • Assess the quality of a ChIP-seq experiment
  • Extract coverage files
  • Call enriched regions or peaks

Licence: Creative Commons Attribution 4.0 International

Keywords: ChIP-seq, Epigenetics

Target audience: Students

Resource type: e-learning

Version: 12

Status: Active

Prerequisites:

  • Introduction to Galaxy Analyses
  • Mapping
  • Quality Control

Learning objectives:

  • Inspect the read quality
  • Trim low quality bases
  • Map reads on a reference genome
  • Assess the quality of a ChIP-seq experiment
  • Extract coverage files
  • Call enriched regions or peaks

Date modified: 2023-11-09

Date published: 2018-09-17

Authors: Anika Erxleben, Bérénice Batut, Fidel Ramirez, Friederike Dündar, Leily Rabbani, Pavankumar Videm, Vivek Bhardwaj

Scientific topics: Epigenomics


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