Abstract

Annotating the eukaryotic genome represents a somewhat more complex challenge than that of prokaryotes, mainly due to the generally larger size of eukaryotic genomes and their greater number of genes, but also to the complexity of eukaryotic genes structure (e.g. exons and Untranslated region (UTR)). This annotation can be carried out at different levels of precision, ranging from simple identification of coding and non-coding parts to detailed structural labeling, including for example the precise location of exons, introns and other regulatory elements.

About This Material

This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.

Questions this will address

• How to annotate an eukaryotic genome with Helixer?
• How to evaluate and visualize annotated genomic features?

Learning Objectives

• Load genome into Galaxy
• Annotate genome with Helixer
• Evaluate annotation quality with BUSCO
• View annotations in JBrowse