e-learning
Genome Assembly of MRSA from Oxford Nanopore MinION data (and optionally Illumina data)
Abstract
Sequencing (determining of DNA/RNA nucleotide sequence) is used all over
About This Material
This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.
Questions this will address
- How to check the quality of the MinION data (together with Illumina data)?
- How to perform an assembly of a bacterial genome with MinION data?
- How to check the quality of an assembly?
Learning Objectives
- Run tools to evaluate sequencing data on quality and quantity
- Process the output of quality control tools
- Improve the quality of sequencing data
- Run a tool to assemble a bacterial genome using short reads
- Run tools to assess the quality of an assembly
- Understand the outputs of tools to assess the quality of an assembly
Licence: Creative Commons Attribution 4.0 International
Keywords: Assembly, amr, assembly, gmod, jbrowse1, microgalaxy, nanopore
Target audience: Students
Resource type: e-learning
Version: 14
Status: Active
Prerequisites:
- Introduction to Galaxy Analyses
- Quality Control
Learning objectives:
- Run tools to evaluate sequencing data on quality and quantity
- Process the output of quality control tools
- Improve the quality of sequencing data
- Run a tool to assemble a bacterial genome using short reads
- Run tools to assess the quality of an assembly
- Understand the outputs of tools to assess the quality of an assembly
Date modified: 2024-03-13
Date published: 2021-03-24
Contributors: Bazante Sanders, Helena Rasche, Miaomiao Zhou, Saskia Hiltemann
Scientific topics: Sequence assembly, Whole genome sequencing, Public health and epidemiology, Genomics, Microbiology, Antimicrobial resistance
External resources:
Activity log
