e-learning

Genome assembly using PacBio data

Abstract

In this tutorial, we will assemble a genome of a species of fungi in the family Mucoraceae, Mucor mucedo, from PacBio sequencing data. These data were obtained from NCBI (SRR8534473, SRR8534474 and SRR8534475). The quality of the assembly obtained will be analyzed, in particular by comparing it to a reference assembly, obtained with Falcon assembler, and available on the JGI website.

About This Material

This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.

Questions this will address

  • How to perform a genome assembly with PacBio data ?
  • How to check assembly quality ?

Learning Objectives

  • Assemble a Genome with PacBio data
  • Assess assembly quality

Licence: Creative Commons Attribution 4.0 International

Keywords: Assembly, assembly, pacbio

Target audience: Students

Resource type: e-learning

Version: 9

Status: Active

Prerequisites:

  • Introduction to Galaxy Analyses
  • Quality Control

Learning objectives:

  • Assemble a Genome with PacBio data
  • Assess assembly quality

Date modified: 2024-10-15

Date published: 2021-11-29

Authors: Alexandre Cormier, Anthony Bretaudeau, Erwan Corre, Laura Leroi, Stéphanie Robin

Scientific topics: Sequence assembly


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