Abstract

Sequencing produces a collection of sequences without genomic context. We do not know to which part of the genome the sequences correspond to. Mapping the reads of an experiment to a reference genome is a key step in modern genomic data analysis. With the mapping the reads are assigned to a specific location in the genome and insights like the expression level of genes can be gained.

About This Material

This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.

Questions this will address

• What is mapping?
• What two things are crucial for a correct mapping?
• What is BAM?

Learning Objectives

• Run a tool to map reads to a reference genome
• Explain what is a BAM file and what it contains
• Use genome browser to understand your data