e-learning

Identification of the binding sites of the Estrogen receptor

Abstract

This exercise uses the dataset from the Nature publication by Ross-Inness et al., 2012.

About This Material

This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.

Questions this will address

  • How is raw ChIP-seq data processed and analyzed?
  • What are the binding sites of the Estrogen receptor?

Learning Objectives

  • Inspect read quality with FastQC
  • Map reads with Bowtie2
  • Assess the quality of an ChIP-seq experiments
  • Extract coverage files
  • Call enriched regions or peaks

Licence: Creative Commons Attribution 4.0 International

Keywords: ChIP-seq, Epigenetics, work-in-progress

Target audience: Students

Resource type: e-learning

Version: 9

Status: Draft

Prerequisites:

  • Introduction to Galaxy Analyses
  • Mapping
  • Quality Control

Learning objectives:

  • Inspect read quality with FastQC
  • Map reads with Bowtie2
  • Assess the quality of an ChIP-seq experiments
  • Extract coverage files
  • Call enriched regions or peaks

Date modified: 2024-08-09

Date published: 2017-09-18

Authors: Anika Erxleben, Bérénice Batut, Fidel Ramirez, Friederike Dündar, Vivek Bhardwaj

Contributors: Björn Grüning, Bérénice Batut, Fidel Ramirez, Helena Rasche, Maria Doyle, Niall Beard, Nicola Soranzo, Saskia Hiltemann

Scientific topics: Epigenomics


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