e-learning
Identification of the binding sites of the Estrogen receptor
Abstract
This exercise uses the dataset from the Nature publication by Ross-Inness et al., 2012.
About This Material
This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.
Questions this will address
- How is raw ChIP-seq data processed and analyzed?
- What are the binding sites of the Estrogen receptor?
Learning Objectives
- Inspect read quality with FastQC
- Map reads with Bowtie2
- Assess the quality of an ChIP-seq experiments
- Extract coverage files
- Call enriched regions or peaks
Licence: Creative Commons Attribution 4.0 International
Keywords: ChIP-seq, Epigenetics, work-in-progress
Target audience: Students
Resource type: e-learning
Version: 9
Status: Draft
Prerequisites:
- Introduction to Galaxy Analyses
- Mapping
- Quality Control
Learning objectives:
- Inspect read quality with FastQC
- Map reads with Bowtie2
- Assess the quality of an ChIP-seq experiments
- Extract coverage files
- Call enriched regions or peaks
Date modified: 2024-08-09
Date published: 2017-09-18
Contributors: Björn Grüning, Bérénice Batut, Fidel Ramirez, Helena Rasche, Maria Doyle, Niall Beard, Nicola Soranzo, Saskia Hiltemann
Scientific topics: Epigenomics
Activity log