e-learning

Identifying Mycorrhizal Fungi from ITS2 sequencing using LotuS2

Abstract

Introduction

About This Material

This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.

Questions this will address

  • What is the fungal community composition in a given soil sample?

Learning Objectives

  • Understand the files needed for running LotuS2
  • Learn to use datasets from Zenodo in a Galaxy tool
  • Learn to upload data files to Galaxy
  • Learn to run the Galaxy LotuS2 tool and what the parameters mean
  • Understand the output files from LotuS2
  • Understand the structure of the mapping file needed by LotuS2 to link sample metadata to a pair of FASTQ files

Licence: Creative Commons Attribution 4.0 International

Keywords: Microbiome, ecology, fungi

Target audience: Students

Resource type: e-learning

Version: 2

Status: Active

Prerequisites:

  • Galaxy Basics for everyone
  • Introduction to Galaxy Analyses

Learning objectives:

  • Understand the files needed for running LotuS2
  • Learn to use datasets from Zenodo in a Galaxy tool
  • Learn to upload data files to Galaxy
  • Learn to run the Galaxy LotuS2 tool and what the parameters mean
  • Understand the output files from LotuS2
  • Understand the structure of the mapping file needed by LotuS2 to link sample metadata to a pair of FASTQ files

Date modified: 2024-09-30

Date published: 2024-09-30

Authors: Sujai Kumar

Contributors: Bethan Manley, Helena Rasche, Paul Zierep, Saskia Hiltemann, Sujai Kumar

Scientific topics: Metagenomics, Microbial ecology


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