e-learning

Making sense of a newly assembled genome

Abstract

In this tutorial we begin with a new genome assembly just produced in the Unicycler tutorial. This is an assembly of E. coli C, which we will be comparing to assemblies of all other complete genes of this species.

About This Material

This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.

Questions this will address

  • I just assembled a genome. How does it compare with already sequenced genomes?
  • How do I find rearranged, inserted, or deleted regions?

Learning Objectives

  • Identification of the most closely related genome to my new assembly
  • Perform sequence comparison to locate rearrangements
  • Identify genes located in deletions

Licence: Creative Commons Attribution 4.0 International

Keywords: Assembly, gmod, jbrowse1, microgalaxy, prokaryote

Target audience: Students

Resource type: e-learning

Version: 29

Status: Active

Prerequisites:

  • Introduction to Galaxy Analyses
  • Quality Control
  • Unicycler Assembly

Learning objectives:

  • Identification of the most closely related genome to my new assembly
  • Perform sequence comparison to locate rearrangements
  • Identify genes located in deletions

Date modified: 2024-06-24

Date published: 2018-06-14

Authors: Anton Nekrutenko, Delphine Lariviere, Helena Rasche

Scientific topics: Sequence assembly, Genomics, Microbiology


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