Abstract

Verification is an essential step in the process of analyzing neoantigens to ensure that the identified peptides or proteins are accurate and reliable. Neoantigens, which are novel antigens arising from tumor-specific mutations, are crucial for cancer immunotherapy. However, the prediction and identification of these neoantigens must be rigorously validated to ensure they are truly present and capable of eliciting an immune response. Without proper verification, the therapeutic potential of neoantigens cannot be realized, leading to wasted resources and failed treatments.

About This Material

This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.

Questions this will address

• How can neoantigens be verified using bioinformatics tools?
• What is the role of mass spectrometry and peptide sequence databases in neoantigen discovery?

Learning Objectives

• Understand the workflow for neoantigen validation.
• Apply bioinformatics tools to validate peptides and proteins.
• Interpret the results from various analytical steps.