e-learning
Peptide and Protein ID using SearchGUI and PeptideShaker
Abstract
Identifying the proteins contained in a sample is an important step in any proteomic experiment. However, in most experimental set ups, proteins are digested to peptides before the LC-MS/MS analysis. In this so-called "bottom-up" procedure, only peptide masses are measured. Therefore, protein identification cannot be performed directly from raw data, but is a multi-step process:
About This Material
This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.
Questions this will address
- How to convert LC-MS/MS raw files?
- How to identify peptides?
- How to identify proteins?
- How to evaluate the results?
Learning Objectives
- Protein identification from LC-MS/MS raw files.
Licence: Creative Commons Attribution 4.0 International
Keywords: DDA, HeLa, Proteomics
Target audience: Students
Resource type: e-learning
Version: 27
Status: Active
Prerequisites:
Introduction to Galaxy Analyses
Learning objectives:
- Protein identification from LC-MS/MS raw files.
Date modified: 2023-11-09
Date published: 2017-06-12
Contributors: Björn Grüning, Bérénice Batut, Clemens Blank, Florian Christoph Sigloch, Helena Rasche, Melanie Föll, Niall Beard, Nicola Soranzo, Saskia Hiltemann, Subina Mehta, William Durand
Scientific topics: Proteomics
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