e-learning
Peptide and Protein Quantification via Stable Isotope Labelling (SIL)
Abstract
To compare protein amounts in different samples from MS/MS data, two different experiment setups exist. Firstly, unmodified proteins can be measured in separate runs at one sample per MS-run. Secondly, proteins of samples to compare can be labelled with small chemical tags, mixed, and measured side-by-side in a single MS-run.
About This Material
This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.
Questions this will address
- What are MS1 features?
- How to quantify based on MS1 features?
- How to map MS1 features to MS2 identifications?
- How to evaluate and optimize the results?
Learning Objectives
- MS1 feature quantitation and mapping of quantitations to peptide and protein IDs.
Licence: Creative Commons Attribution 4.0 International
Keywords: DDA, Proteomics, SILAC
Target audience: Students
Resource type: e-learning
Version: 31
Status: Active
Prerequisites:
- Introduction to Galaxy Analyses
- Peptide and Protein ID using OpenMS tools
- Protein FASTA Database Handling
Learning objectives:
- MS1 feature quantitation and mapping of quantitations to peptide and protein IDs.
Date modified: 2023-11-09
Date published: 2017-03-17
Scientific topics: Proteomics
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