e-learning
Proteogenomics 2: Database Search
Abstract
In this tutorial, we perform proteogenomic database searching using the Mass Spectrometry data. The inputs for performing the proteogenomic database searching are the peaklist MGF files and the FASTA database file. The FASTA database is obtained by running the first workflow “UniprotcRAPSAVindeltranslatedbed.FASTA”. The second workflow focuses on performing database search of the peak list files (MGFs).
About This Material
This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.
Questions this will address
- How to identify variant proteoforms in MS data by searching with the customized Protein database?
Learning Objectives
- A proteogenomic data analysis of mass spectrometry data to identify and visualize variant peptides.
Licence: Creative Commons Attribution 4.0 International
Keywords: Proteomics, proteogenomics
Target audience: Students
Resource type: e-learning
Version: 23
Status: Active
Prerequisites:
- Introduction to Galaxy Analyses
- Proteogenomics 1: Database Creation
Learning objectives:
- A proteogenomic data analysis of mass spectrometry data to identify and visualize variant peptides.
Date modified: 2024-08-09
Date published: 2018-11-20
Scientific topics: Proteomics
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