e-learning

Quality and contamination control in bacterial isolate using Illumina MiSeq Data

Abstract

Sequencing (determining of DNA/RNA nucleotide sequence) is used all over the world for all kinds of analysis. The product of these sequencers are reads, which are sequences of detected nucleotides. Depending on the technique these have specific lengths (30-500bp) or using Oxford Nanopore Technologies sequencing have much longer variable lengths.

About This Material

This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.

Questions this will address

  • How to check the quality of MiSeq data?
  • What are the species in bacterial isolate sequencing data?

Learning Objectives

  • Run tools to evaluate sequencing data on quality and quantity
  • Evaluate the output of quality control tools
  • Improve the quality of sequencing data
  • Run a series of tool to identify species in bacterial isolate sequencing data
  • Visualize the species abundance

Licence: Creative Commons Attribution 4.0 International

Keywords: Sequence analysis, bacteria, illumina, microgalaxy

Target audience: Students

Resource type: e-learning

Version: 3

Status: Active

Prerequisites:

Introduction to Galaxy Analyses

Learning objectives:

  • Run tools to evaluate sequencing data on quality and quantity
  • Evaluate the output of quality control tools
  • Improve the quality of sequencing data
  • Run a series of tool to identify species in bacterial isolate sequencing data
  • Visualize the species abundance

Date modified: 2024-10-09

Date published: 2024-07-15

Authors: Bérénice Batut, Clea Siguret

Scientific topics: Sequence analysis, Whole genome sequencing, Genomics, Microbiology, Microbial ecology


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