e-learning

Refining Genome Annotations with Apollo (prokaryotes)

Abstract

After automatically annotating your genome using Prokka for example, it is important to visualize your results so you can understand what your organism looks like, and then to manually refine these annotations along with any additional data you might have. This process is most often done as part of a group, smaller organisms may be annotated individually though.

About This Material

This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.

Questions this will address

  • How to visualize your genome after automated annotations have been performed?
  • How to manually annotate genome after automated annotations have been performed?
  • How to evaluate and visualize annotated genomic features?
  • How do I collaborate when doing genome annotation?

Learning Objectives

  • Load a genome into Galaxy
  • View annotations in JBrowse
  • Learn how to load JBrowse data into Apollo
  • Learn how to manually refine genome annotations within Apollo
  • Export refined genome annotations

Licence: Creative Commons Attribution 4.0 International

Keywords: Genome Annotation, apollo2, gmod, jbrowse1, microgalaxy, prokaryote

Target audience: Students

Resource type: e-learning

Version: 20

Status: Active

Prerequisites:

  • Genome annotation with Prokka
  • Introduction to Galaxy Analyses
  • Rule Based Uploader

Learning objectives:

  • Load a genome into Galaxy
  • View annotations in JBrowse
  • Learn how to load JBrowse data into Apollo
  • Learn how to manually refine genome annotations within Apollo
  • Export refined genome annotations

Date modified: 2024-06-14

Date published: 2021-06-04

Authors: Anthony Bretaudeau, Helena Rasche, Mateo Boudet, Nathan Dunn

Scientific topics: Genomics, Microbiology, Gene and protein families, Sequence analysis


Activity log