e-learning

Taxonomic Profiling and Visualization of Metagenomic Data

Abstract

The term "microbiome" describes "a characteristic microbial community occupying a reasonably well-defined habitat which has distinct physio-chemical properties. The term thus not only refers to the microorganisms involved but also encompasses their theatre of activity".

About This Material

This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.

Questions this will address

  • Which species (or genera, families, ...) are present in my sample?
  • What are the different approaches and tools to get the community profile of my sample?
  • How can we visualize and compare community profiles?

Learning Objectives

  • Explain what taxonomic assignment is
  • Explain how taxonomic assignment works
  • Apply Kraken and MetaPhlAn to assign taxonomic labels
  • Apply Krona and Pavian to visualize results of assignment and understand the output
  • Identify taxonomic classification tool that fits best depending on their data

Licence: Creative Commons Attribution 4.0 International

Keywords: Microbiome, metagenomics, microgalaxy, taxonomic profiling

Target audience: Students

Resource type: e-learning

Version: 4

Status: Active

Prerequisites:

Introduction to Galaxy Analyses

Learning objectives:

  • Explain what taxonomic assignment is
  • Explain how taxonomic assignment works
  • Apply Kraken and MetaPhlAn to assign taxonomic labels
  • Apply Krona and Pavian to visualize results of assignment and understand the output
  • Identify taxonomic classification tool that fits best depending on their data

Date modified: 2024-06-14

Date published: 2023-05-03

Authors: Bérénice Batut, Paul Zierep, Sophia Hampe

Scientific topics: Metagenomics, Microbial ecology, Taxonomy, Sequence analysis


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