Tutorial

Transcriptomics - CLIP-Seq data analysis from pre-processing to motif detection

Training material for all kinds of transcriptomics analysis.

Questions of the tutorial:

  • How is raw CLIP-Seq data processed and analysed?
  • How do I find binding motifs and targets for a protein (e.g., RBFOX2)?

Objectives of the tutorial:

  • Remove Adapters, Barcodes and Unique Molecular Identifiers (UMIs) from the reads
  • Align trimmed reads with STAR
  • De-duplicate the read library
  • Inspect the read mapping and de-duplication quality
  • Perform peak calling with PEAKachu
  • Analyse the peaks and find potential binding motifs and targets
  • Check the quality of the peak calling

Resource type: Tutorial

Authors: Florian-H-Lab, dmaticzka, bebatut

External resources:

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