Abstract

Heatmaps are commonly used to visualize RNA-Seq results. They are useful for visualizing the expression of genes across the samples. In this tutorial we show how the heatmap2 tool in Galaxy can be used to generate heatmaps. The heatmap2 tool uses the heatmap.2 function from the R gplots package. Here we will demonstrate how to make a heatmap of the top differentially expressed (DE) genes in an RNA-Seq experiment, similar to what is shown for the fruitfly dataset in the RNA-seq ref-based tutorial. We will also show how a heatmap for a custom set of genes an be created.

About This Material

This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.

Questions this will address

• How to generate heatmaps from RNA-seq data?

Learning Objectives

• Create heatmaps of RNA-seq data