Abstract

As sessile organisms, the survival of plants under adverse environmental conditions depends, to a large extent, on their ability to perceive stress stimuli and respond appropriately to counteract the potentially damaging effects. Coordination of phytohormones and reactive oxygen species are considered a key element for enhancing stress resistance, allowing fine-tuning of gene expression in response to environmental changes,. These molecules constitute complex signalling networks, endowing with the ability to respond to a variable natural environment.

About This Material

This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.

Questions this will address

• Which miRNAs are upregulated in response to brassinosteroids?
• Which genes are potential target of brassinosteroid-induced miRNAs?

Learning Objectives

• Perform miRNA differential expression analysis
• Understand the quasi-mapping-based Salmon method for quantifying the expression of transcripts using RNA-Seq data
• Idenfity potential miRNAs involved in brassinosteroid-mediated regulation networks