This workshop will introduce open-source tools for creating, visualizing and analysing biological networks oriented towards the study of pathogens. Training will be provided on computational tools and databases such as Reactome, Cytoscape, Gephi for graph visualization and analyses, MetaFlux, Pathway Tools, BioCyc for reconstruction of pathway/genome databases using omics data, and Target Pathogen for the discovery of targets using integrative approaches. The main principles of network analysis will be covered as well as its applications to health and disease.