Many Latin American countries are hotspots for biodiversity, most of which have been characterized only to a very limited extent. This course aims to provide knowledge on available approaches, methods and tools to perform studies on biodiversity employing modern high-throughput sequencing approaches. After the course, participants will have learnt how to select the appropriate sequencing approach and experimental setup, how to access, control quality and clean data, and to analyze and interpret results using available bioinformatics tools.