Polygenic scores (PGS) are a measurement which represents genetic predisposition for a heritable trait or phenotype by aggregating the effects of hundreds-to-millions of genetic variants into a single number. The PGS Catalog is the world’s largest FAIR (finable, accessible, interoperable, and reusable) repository of PGS along with the relevant metadata required to evaluate and reuse them. PGS have emerged as a commonly used genomic tool that have multiple research applications and potential clinical applications. This webinar will demonstrate how to use scoring files from the PGS Catalog (or custom files) to calculate PGS in your own samples using the PGS Catalog Calculator (pgsccalc). The pgsccalc software reproducibly automates PGS calculation in commonly used genotyping formats (plink, VCF) alongside capabilities for adjusting PGS in the context of genetic ancestry (necessary for proper interpretation of PGS across diverse populations). This webinar introduces the software and describes the ancestry adjustment process included in the Calculator, along with information about how to run the tool and interpret results.