UniProt KnowledgeBase (UniProtKB) supports biomedical research by providing a comprehensive, high-quality, and freely accessible resource of protein sequence and functional information. This webinar will provide an overview on how to access human disease and protein variant information available in UniProtKB. It will outline the annotation of protein molecular function, involvement in human disease, as well as disease-associated variants, including variant position, variant characterisation and their impact on protein structure and function. It will present the protein variant viewer and feature viewer and demonstrate how the feature viewer facilitates visualisation of variant impact on protein features and 3D structure. We will discuss the cross-links to human-specific databases and tools such as BLAST and Align. In addition, we will also demonstrate how to access and download human variation datasets available in UniProtKB. By the end of this webinar, attendees will have a solid foundation in navigating UniProtKB to access disease-related protein data and utilising the various protein molecular data to analyse the impact of protein variants on protein function and structure.