Here, we have made some of the Proteomics bioinformatics courses' materials available for you to access any time. The last edition of the Proteomics bioinformatics course ran in July 2024.This set of materials goes through the basics of mass spectrometry (MS) and proteomics bioinformatics. The materials include: how to use search engines and post-processing software, quantitative approaches, MS data repositories, the use of public databases for protein analysis, annotation of subsequent protein lists, and incorporation of information from molecular interaction and pathway databases.This course is organised in association with the Vlaams Instituut voor Biotechnologie (VIB).