UniProt provides the scientific community with a freely accessible collection of protein sequences and functional information and is one of the most widely used protein resources in the world. BLAST and the multiple sequence alignment are essential methods commonly used to find and compare protein or nucleotide sequences. In this webinar, we will demonstrate how you can access and run these sequence analysis tools in UniProt. We will show you how to navigate the results and how you can exploit UniProt protein annotation to expand further the analysis of your results.